The planned schedule for the May 2023 Data Lab Virtual Training Workshop “Introduction to Single-Cell RNA-Seq” appears below.

Note: All times are EDT (UTC−04:00).

Time Topic Location
Day 1 2023-05-15
Introduction to R and the Tidyverse
 
12:00 PM Welcome, Introductions and Getting Started
Workshop introduction slides (PDF)
Zoom: Main Session
1:00 PM Introduction to base R
Introduction to R, RStudio, and RStudio Server slides (PDF)
Zoom: Main Session
2:30 PM Introduction to ggplot2 Zoom: Main Session
3:30 PM Introduction to the tidyverse Zoom: Main Session
4:30 PM Questions and introduction to the exercises Zoom: Breakout Rooms
  Exercise: Introduction to base R  
  Exercise: Introduction to R  
  Exercise: Introduction to the tidyverse, Part 1  
  Exercise: Introduction to the tidyverse, Part 2  
  Consultation session and exercises Slack channel
5:00 PM Adjourn for the day  
     
Day 2 2023-05-16
Introduction to Single-cell RNA-seq, Day 1
 
12:00 PM Introduction
Introduction to scRNA-seq slides (PDF)
Zoom: Main Session
12:30 PM scRNA-seq quantification with Alevin Zoom: Main Session
1:30 PM Importing data and filtering cells Zoom: Main Session
2:30 PM Normalization of expression data Zoom: Main Session
3:30 PM Questions and introduction to the exercise Zoom: Breakout Rooms
  Exercise: scRNA-seq quantification  
  Exercise and consultation session Slack channel
5:00 PM Adjourn for the day  
     
Day 3 2023-05-17
Introduction to Single-cell RNA-seq, Day 2
 
12:00 PM Dimension reduction & visualization
Dimension reduction and clustering slides (PDF)
Zoom: Main Session
1:30 PM Clustering and marker identification Zoom: Main Session
3:00 PM Questions and introduction to the exercise Zoom: Breakout Rooms
  Exercise: scRNA-seq clustering  
  Exercise and consultation session Slack channel
5:00 PM Adjourn for the day  
     
Day 4 2023-05-18
Introduction to Single-cell RNA-seq, Day 3
 
12:00 PM Cell type annotation
Cell type annotation slides (PDF)
Zoom: Main Session
2:30 PM Questions and introduction to the exercise Zoom: Breakout Rooms
  Exercise and consultation session Slack channel
  Exercise: scRNA-seq cell type annotation  
5:00 PM Adjourn for the day  
     
Day 5 2023-05-19
Consultation and Presentations
 
12:00 PM Consultation session Slack channel
2:30 PM Participant presentations begin Zoom: Main Session
5:00 PM Adjourn