Advanced scRNA-seq Workshop Schedule
The planned schedule for the June 10-12, 2025 Data Lab Training Workshop appears below.
| Time | Topic |
|---|---|
| Day 1 | 2025-06-10 |
| 9:00 AM | Welcome, Introductions and Getting Started |
| 10:00 AM | Introduction/Refresher to Single-cell Analysis with R and Bioconductor |
| Single-cell Analysis with R slides (PDF) | |
| Instruction notebook | |
| 11:15 AM | Coffee break |
| 11:30 AM | Introduction/Refresher to Single-cell Analysis, continued |
| 12:15 PM | Lunch |
| 1:15 PM | Integrating scRNA-seq Experiments |
| Integration slides (PDF) | |
| Instruction notebook | |
| 2:30 PM | Coffee break |
| 2:45 PM | Integrating scRNA-seq Experiments, continued |
| 4:15 PM | Exercises and Questions |
| Exercise: Importing CITE-seq data | |
| Exercise: Cell type assignment This exercise is available in the scRNA-seq module |
|
| Exercise: Integration | |
| 5:00 PM | Adjourn for the day |
| Day 2 | 2025-06-11 |
| 9:00 AM | Questions and Review |
| 9:30 AM | Differential Expression Analysis with scRNA-seq |
| Differential Expression slides (PDF) | |
| Instruction notebook | |
| 11:00 AM | Coffee break |
| 11:15 AM | Differential Expression Analysis, continued |
| 12:30 PM | Lunch |
| 1:30 PM | Exercises, Questions, and Independent Projects |
| Exercise: Differential Expression | |
| Resources for consultation sessions | |
| 4:00 PM | Adjourn for the day |
| 6:00 PM | Workshop dinner: Manayunk Brewing Company 4120 Main St, Philadelphia PA 19127 |
| Day 3 | 2025-06-12 |
| 9:00 AM | Questions and Review |
| 9:30 AM | Pathway Analysis |
| Pathway Analysis Slides (PDF) | |
| Instruction notebook: Gene Set Enrichment Analysis (GSEA) | |
| 11:00 AM | Coffee break |
| 11:15 AM | Instruction notebook: AUCell |
| 12:30 PM | Lunch |
| 1:30 PM | Exercises, Questions, and Independent Projects |
| Exercise: AUCell | |
| Exercise: Evaluating cluster quality | |
| Resources for consultation sessions | |
| 2:45 PM | An Introduction to the OpenScPCA Project |
| 3:00 PM | Participant Presentations! |
| 4:00 PM | Adjourn |